24 hr-survival after a single 3H-thymidine injection on E19 (lateral sagittal)

Figure 108.  This lateral sagittal slice is through the olfactory bulb of the same specimen in Figure 107.  The olfactory recess is narrowing, and we are at its lateral edge—still there is a definite place where cells are spilling into the ventricle from the olfactory NEP—a characteristic of E20 specimens.  After a 3H-thymidine exposure on the morning of E19, most labeled cells are still in the NEP and SVZ rather than in the surrounding layers piling up in the parenchyma. This slice shows similar layers as the medial slice, only the AOB forms a larger “hump” in the dorsal bulb, and the AOB granule cell layer takes up more room.  In the MOB, the features of layers and labeling is nearly identical to the medial slice (Fig. 107).  Heavy label uptake in the olfactory epithelium shows very active proliferation, and the superficial and deep separation of labeled cells is clear.